Estimation of Pioglitazone Hydrochloride and Alogliptin Benzoate in Combination by Second Order Derivative Spectrophotometery Method

Abstract

Diabetes mellitus, a disease characterized by hyperglycaemia is spreading like epidemic in most of the developing and developed countries. A simple and accurate method for analysis of Pioglitazone Hydrochloride (PIO) and Alogliption benzoate (ALO) in their combination was developed using second order derivative spectrophotometry. PIO and ALO were quantified using second derivative responses at 267 nm and 278 nm prepared in methanol. The calibration curves were found to be linear in the concentration range of 10-30 µg/mL for both PIO and ALO. The method was validated as per ICH guideline and found to be accurate and precise. Developed method was successfully applied for the estimation of PIO and ALO in their synthetic mixture.